Separation of monoclonal antibodies from antihapten antisera by two-dimensional affinity electrophoresis. |
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Authors: | K Takeo K Nakamura R Suzuno |
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Institution: | First Department of Biochemistry, Yamaguchi University School of Medicine, Ube, Japan. |
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Abstract: | A high-resolution two-dimensional affinity electrophoresis (2D-AEP) method was developed, using capillary polyacrylamide gel (PAG) isoelectric focusing in the first and slab PAG affinity electrophoresis in the second direction. Using this method, anti-hapten antibodies were separated into a number of monoclonal antibody immunoglobulin G (IgG)] families, each of which is composed of several IgG spots having an identical affinity to the hapten but different isoelectric points. 2D-AEP may offer a powerful tool for solving fundamental problems in immunochemistry such as antibody heterogeneity, its hapten binding specificity and antigen-dependent somatic mutation. |
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