| A fluorescence enhanced probe for sulfur dioxide detection and fluorescence imaging in living cellsEI北大核心CSCD |
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| 引用本文： | 王敏,张静怡,刘娜,张迪. A fluorescence enhanced probe for sulfur dioxide detection and fluorescence imaging in living cellsEI北大核心CSCD[J]. 分析试验室, 2023, 0(8): 1094-1099 |
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| 作者姓名： | 王敏 张静怡 刘娜 张迪 |
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| 作者单位： | 1. 郑州师范学院化学化工学院;2. 河南省农业科学院农业质量标准与检测技术研究所 |
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| 基金项目： | 河南省科技攻关项目（222102310384）; |
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| 摘 要： | 基于香豆素和苯并吡啶基团,构建了用于二氧化硫(SO_(2))高效检测的荧光探针P1,其化学结构通过核磁氢谱(^(1)H NMR)、碳谱(^(13)C NMR)和高分辨质谱(HR-MS)确证。在缓冲溶液体系中,单独的探针P1具有微弱的荧光,识别SO_(2)后荧光发射强度明显增强,能够实现对SO_(2)的专一性裸眼识别,检出限为126 nmol/L。生物应用实验结果表明,该探针具有较低的细胞毒性,可用于生物活细胞中外源性SO_(2)的荧光成像。
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| 关 键 词： | 二氧化硫 荧光探针 荧光增强 荧光成像 |
A fluorescence enhanced probe for sulfur dioxide detection and fluorescence imaging in living cells |
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| Wang,Min,Zhang,Jingyi,Liu,Na,Zhang,Di. A fluorescence enhanced probe for sulfur dioxide detection and fluorescence imaging in living cells[J]. Chinese Journal of Analysis Laboratory, 2023, 0(8): 1094-1099 |
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| Authors: | Wang Min Zhang Jingyi Liu Na Zhang Di |
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| Affiliation: | 1.College of Chemistry and Chemical Engineering, Zhengzhou Normal University, Zhengzhou,450044, China;2.Institute of Agricultural Quality Standards and Testing Technology, Henan Academy of Agricultural Sciences, Zhengzhou,450052, China; |
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| Abstract: | A fluorescent probe P1 was constructed for efficient detection of sulfur dioxide (SO2) based on coumarin and benzopyran groups. Its chemical structure was confirmed by 1hydrogen-nuclear magnetic resonance (1H NMR), 13carbon-nuclear magnetic resonance (13C NMR) and high resolution mass spectrometry (HR-MS). In buffer solution system, the single probe P1 showed very weak fluorescence, and the fluorescence emission intensity significantly enhanced after the recognition of SO2, which could realize the specific naked eye recognition of SO2 with the detection limit of 126 nmol/L. The results of biological application experiments indicated that the probe had low cytotoxicity and could be used for fluorescence imaging of exogenous SO2 in living biological cells. © 2023, Youke Publishing Co.,Ltd. All rights reserved. |
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| Keywords: | Mass spectrometry - Nuclear magnetic resonance - Probes - Sulfur dioxide |
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