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Determination of uracil in the culture fluid of Glomerella cingulata and infected hosts by high-performance liquid chromatographyEI北大核心CSCD
引用本文:于鑫,邢小霞,董向丽,李平亮.Determination of uracil in the culture fluid of Glomerella cingulata and infected hosts by high-performance liquid chromatographyEI北大核心CSCD[J].分析试验室,2023(8):1013-1019.
作者姓名:于鑫  邢小霞  董向丽  李平亮
作者单位:青岛农业大学植物医学学院,山东省植物病虫害绿色防控工程研究中心
基金项目:国家自然科学基金青年项目(32102253);;山东省自然科学基金青年项目(ZR2020QC137);
摘    要:A CRISPR-Cas system holds great promise as a next-generation biosensing technology for molecular diagnostics. In this paper, a portable biosensor based on the trans-cleavage activity of CRISPR-Cas12a and a personal glucose meter has been developed for quantitative, sensitive and specific detection of melamine. The presence of the target melamine binds to the aptamer, leading to the release of locker DNA. And then, the leasing locker DNA activates the trans-cleavage activity of CRISPR-Cas12a to cleave the single-strand DNA (ssDNA) linker on sucrase-ssDNA modified electrode, releasing a short DNA fragment labeled with sucrase in the resulting solution. The sucrase could further catalyze sucrose to glucose, which could be detected by the PGM. Under the optimized conditions, the increase of PGM signal was relative with the concentration of melamine ranging from 0.1 to 2.5 μmol/L and the limit of detection (LOD) was 37 nmol/L. Moreover, the portable biosensor has strong specificity and can be used for the quantitative detection of melamine in milk samples. © 2023, Youke Publishing Co.,Ltd. All rights reserved.

关 键 词:苹果炭疽叶枯病菌  高效液相色谱法  尿嘧啶  病菌培养液  苹果叶片
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