Determination of hydroxypropyl bis (N-hydroxyethyl-p-phenylenediamine) hydrochloride in hair dye products by HPLC and its verification by LC-MS/MSEI北大核心CSCD |
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引用本文: | 左雪,杨玲,邸铮,邬国庆.Determination of hydroxypropyl bis (N-hydroxyethyl-p-phenylenediamine) hydrochloride in hair dye products by HPLC and its verification by LC-MS/MSEI北大核心CSCD[J].分析试验室,2023(4):540-544. |
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作者姓名: | 左雪 杨玲 邸铮 邬国庆 |
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作者单位: | 北京市药品检验研究院 |
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摘 要: | A method for the determination of hydroxypropyl bis (N-hydroxyethyl-p-phenylenediamine)hydrochloride in hair dye products by high performance liquid chromatography (HPLC) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed. The sample was ultrasonically extracted with 50% (V/V) ethanol. Chromatographic separation was carried out on a Waters Symmetry C18 column (250 mm×4.6 mm, 5 µm) by isocratic elution. The mobile phase was 0.02 mol/L ammonium acetate aqueous solution and acetonitrile (84∶16, V/V). The detection wavelength was 258 nm. Quantification was performed by external standard method. Verification for positive samples was performed by LC-MS/MS. The results showed good linearity from 2 mg/L to 200 mg/L, with the correlation coefficients (r) higher than 0.999. The limit of detection (LOD) was 0.003%(w/w), and the limit of quantification (LOQ) was 0.01%(w/w). The average spiked recoveries at three levels in both cream and liquid substrates were from 97.5% to 103.4%, with the relative standard deviations (RSDs) from 1.7% to 3.9%. The method could be used to detect hydroxypropyl bis (N1]hydroxyethyl-p-phenylenediamine)hydrochloride in hair dye products, which could fill the gap of the current standard. © 2023, Youke Publishing Co.,Ltd. All rights reserved.
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关 键 词: | 羟丙基双(N-羟乙基对苯二胺)盐酸盐 染发产品 高效液相色谱法 液相色谱-串联质谱法 |
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