Studies on temperature dependent kinetics of <Emphasis Type="Italic">Aspergillus awamori</Emphasis> feruloyl esterase in water solutions |
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Authors: | A E Fazary S Ismadji Y H Ju |
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Institution: | 1.Department of Chemical Engineering,National Taiwan University of Science and Technology,Taipei,Taiwan |
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Abstract: | The initial reaction rate (V
0) for the esterification reaction of feruloyl esterase (FAE-II) at different temperatures (288, 298, 308, 318, 328, 338, 348,
and 358 K) and various ethyl ferulate concentrations (2, 4, 6, 8, 10, 12, 14, and 16) × 10−4 mol l−1 of ethyl ferulate in water] were determined. The Lineweaver-Burk double reciprocal plot yielded the kinetic parameters (maximal
velocity V
max, Michaelis constant K
m, and second order rate constant V/K). The effects of temperature on those 3 kinetic parameters were presented and discussed. The thermodynamic parameters ΔH* (enthalpy of activation), ΔG* (free energy of activation), ΔS* (entropy of activation), ΔG
E-S (free energy change of substrate binding), ΔG
E-T (free energy change of transition state formation), related to that biochemical process were determined and discussed from
van’t Hoff plot, Arrhenius plot, and Eyring plot. |
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Keywords: | |
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