Scanning electrochemical microscopy of enzymes immobilized on structured glass-gold substrates |
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| Authors: | T Wilhelm G Wittstock R Szargan |
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| Institution: | University of Leipzig, Wilhelm-Ostwald-Institute of Physical and Theoretical Chemistry, Linnéstrasse 2, D-04103 Leipzig, Germany e-mail: wittstoc@rz.uni-leipzig.de, DE
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| Abstract: | Scanning electrochemical microscopy (SECM) was used to characterize enzyme-modified glass-gold specimens. The exposed gold surface was functionalized with an aminothiol and reacted with carbodiimide-activated glucose oxidase. The specimen surface was examined with SECM, using a 25 μm platinum electrode. Images were acquired showing the topography, electric conductivity, and enzymatic activity of the composite surface. It was found that the hydroxy-groups of the glass surface are as likely to bind to the activated enzyme as the amino-groups on the gold surface. |
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