Simultaneous determination of propanol and butanol by a fluorometric enzymatic method based on the intrinsic fluorescence of the enzyme |
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| Authors: | José F Sierra Marta de Pedro Susana de Marcos J Galbán Juan R Castillo C Ubide |
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| Institution: | GEAS, Analytical Chemistry Department, Faculty of Sciences, University of Zaragoza, E-50009 Zaragoza, Spain, ES
Applied Chemistry Department, Faculty of Chemistry, University of Pais Vasco, Apdo. 1072, E-20080 San Sebastian, Spain, ES
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| Abstract: | The application of the intrinsic fluorescence of alcohol dehydrogenase (ADH) for the simultaneous determination of propanol and butanol was investigated by using only one kinetic run. An absolute calibration method (based on a mathematical model derived from the enzymatic reaction) and a multivariate calibration method (partial least squares regression, PLSR) were tested for this purpose. Both methods were applied to synthetic samples of both alcohols with good results. |
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