| 蛋白质的微乳液毛细管电动色谱分离研究 |
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| 引用本文： | 张宣,胡亚男,王义明,周国华,罗国安.蛋白质的微乳液毛细管电动色谱分离研究[J].高等学校化学学报,2000,21(8):1183-1186. |
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| 作者姓名： | 张宣 胡亚男 王义明 周国华 罗国安 |
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| 作者单位： | 清华大学化学系, 北京 100084 |
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| 基金项目： | 国家自然科学基金! (批准号 :692 3 5 0 2 2 0 ) |
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| 摘 要： | 考察了用微乳液毛细管电动色谱(MEEKC)分离蛋白质时微乳液组成等不同因素对分离的影响,并与胶束电动色谱进行对比,探讨了其分离机理,为蛋白质的分离鉴定提供了一种有力的工具.
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| 关 键 词： | 微乳液毛细管电动色谱 胶束电动色谱 蛋白质 |
| 文章编号： | 0251-0790(2000)08-1183-04 |
| 收稿时间： | 1999-09-28 |
Separation of Proteins by Microemulsion Electrokinetic Chromatography |
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| ZHANG Xuan,HU Ya-Nan,WANG Yi-Ming,ZHOU Guo-Hua,LUO Guo-An.Separation of Proteins by Microemulsion Electrokinetic Chromatography[J].Chemical Research In Chinese Universities,2000,21(8):1183-1186. |
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| Authors: | ZHANG Xuan HU Ya-Nan WANG Yi-Ming ZHOU Guo-Hua LUO Guo-An |
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| Institution: | Department of Chemistry, Tsinghua University, Beijing 100084, China |
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| Abstract: | Microemulsion electrokinetic chromatography(MEEKC) is a new technique which was employed to separate both basic and acidic proteins simultaneously. Optimum separation conditions were investigated with respect to ionic strength and the composition of microemulsion. The results show that if a stable microemulsion formed, the efficiency of protein separation was slightly affected by the concentration of the background and the composition of microemulsion. The fundamental characteristics of MEEKC were studied in comparison with micellar electrokinetic chromatography(MEKC). The mechanism of MEEKC was also investigated. The results show that MEEKC is an alternative way for protein separations with a high efficiency. |
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| Keywords: | Microemulsion electrokinetic chromatography Micellar electrokinetic chromatography Protein |
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