| 高效液相色谱梯度洗脱中弱溶剂的在线净化 |
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| 引用本文： | 马建苹,刘满仓,朱彭龄. 高效液相色谱梯度洗脱中弱溶剂的在线净化[J]. 分析测试技术与仪器, 1999, 5(1): 19-23 |
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| 作者姓名： | 马建苹 刘满仓 朱彭龄 |
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| 作者单位： | 兰州大学化学系 |
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| 基金项目： | 国家自然科学基金资助课题。编号:29457198 |
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| 摘 要： | 利用一个在线净化预柱减少了高压梯度洗脱中由于试剂纯度不够造成的基线噪音。预柱的位置在溶剂泵Ａ和流动相混合器之间，由一个六通阀来控制。在线净化结果良好，有效地除去了空白梯度色谱图中的杂质峰，提高了梯度洗脱的分离重现性。该方法对开展梯度洗脱有实用价值。
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| 关 键 词： | 高效液相色谱 梯度洗脱 预柱 净化 弱溶剂 |
| 收稿时间： | 1998-08-10 |
On-Line Cleanup of Weak Solvent in Gradient Elution |
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| MA Jianping,LIU Mancang and ZHU Pengling. On-Line Cleanup of Weak Solvent in Gradient Elution[J]. Analysis and Testing Technology and Instruments, 1999, 5(1): 19-23 |
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| Authors: | MA Jianping LIU Mancang ZHU Pengling |
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| Affiliation: | Department of Chemistry, Lanzhou University Lanzhou 730000;Department of Chemistry, Lanzhou University Lanzhou 730000;Department of Chemistry, Lanzhou University Lanzhou 730000 |
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| Abstract: | Baseline noise in high-pressure gradient elution caused by impurities in weak aqueous solvent (solvent A) was improved with an on-line cleanup precolumn placed between the solvent pump A (weak solvent) and the mobile phase mixer. The procedure was implemented by using a sixport valve. With the various TEAP, H-2O/ACN, MeOH combinations examined, results obtained in the work indicate that the cleanup method is sufficiently effective and reproducible. |
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| Keywords: | high-performance liquid chromatography gradient elution precolumn on-line cleanup |
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