适用于高通量筛选的氢化可的松生物转化率快速检测方法研究

以浓硫酸显色反应为基础,通过分光光度计可见光双波长法测定氢化可的松转化率。利用朗伯比尔定律,结合三维数据建模二阶校正,建立了氢化可的松生物转化率的快速测定方法,回收率为96.9%~110.8%。验证实验显示,其测定结果与药典方法(高效液相色谱)具有很高的一致性,能够满足菌种高通量初筛检测的需求。该研究在该菌种高通量选育方面有良好的应用前景。

A New Rapid Method for Hydrocortisone Biotransformation Rates Determination Applicable for High-throughput Selection System

In this study,concentrated sulphuric acid was employed for the determination of hydrocortisone biotransformation rate,which could be monitored via dual wavelength analysis.A rapid and simple method for the determination of hydrocortisone biotransformation rate was developed by combining the well-known Lambert Beer law and the 3D data modeling method.The method recoveries were in the range of 96.9%-110.8%.The validation results,which were consistent with that of HPLC method,showed that the method could meet the needs for preliminary screening of Absidia coerulea via high-throughput system.This study has a good application prospect in breeding Absidia coerulea of high specific biotransformation ability.