A study of the antioxidative behavior of phenolic acids, in aqueous herb extracts, using a dsDNA biosensor |
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Authors: | Elina Skeva and Stella Girousi |
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Institution: | (1) Centre of Bioanalysis, National Institute for Biological Science, 296, Spl. Independentei, Bucharest, 060031, Romania |
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Abstract: | Electrochemical DNA biosensors are promising tools for the fast, inexpensive and simple in vitro analysis for the determination of free radicals and antioxidants. High concentrations of antioxidants in such compounds as
phenolic acids and plant extracts, act as free radical terminators which reduce the effect of the oxidative dam-age on DNA.
The electrochemical behavior of three representative phenolic acids, caffeic acid, gallic acid and trolox were studied by
cyclic voltammetry. Moreover, the determination of the above antioxidants under the optimized conditions (scan rate, deposition
potential and time) using differential pulse voltammetry was also investigated. In vitro studies focused on their antioxidative effect were performed by adsorptive transfer stripping voltammetry and dsDNA biosensor.
Using Fenton’s system, with FeSO4 and H2O2 was chosen as a strong oxidative system. This biosensor was applied as a screening antioxidant test in order to estimate
the antioxidant capacity of aqueous herb extracts.
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