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| 单细胞拉曼光谱结合多元统计方法分析不同酿酒酵母菌株的成分差异 | |
| 引用本文： | Lai JZ,Liu B,Wang GW,Tao ZH,Huang SS. 单细胞拉曼光谱结合多元统计方法分析不同酿酒酵母菌株的成分差异[J]. 光谱学与光谱分析, 2011, 31(2): 412-417. DOI: 10.3964/j.issn.1000-0593(2011)02-0412-06 |
| 作者姓名： | Lai JZ Liu B Wang GW Tao ZH Huang SS |
| 作者单位： | 1. 广西科学院,广西,南宁,530003;广西大学生命科学与技术学院,广西,南宁,530004 2. 广西大学农学院应用微生物研究所,广西,南宁,530005 3. 广西科学院,广西,南宁,530003 |
| 基金项目： | 国家自然科学基金项目(30760010); 广西自然科学重点基金项目(2010GXNSFD013029); 广西科学院基本科研业务费项目(09YJ17WL01)资助 |
| 摘 要： | 应用单细胞拉曼光谱技术结合多元统计方法分析比较了六株不同来源的酿酒酵母菌株的同步化细胞,从而获知菌株间细胞成分的差异.利用连续密度梯度离心法分离酿酒酵母同步化细胞,分别对六个酵母菌株的同步化单细胞进行拉曼信号收集,并结合主成分分析(PCA)、辨别函数分析(DFA)两种多元统计分析光谱差异.结果表明,细胞的拉曼光谱表征其... |
| 关 键 词： | 拉曼光谱 多元统计 单细胞 酿酒酵母菌株 |
Analysis of components difference of yeast strains based on laser tweezers Raman spectroscopy combined with multistatistical analysis |
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| Lai Jun-Zhuo,Liu Bin,Wang Gui-Wen,Tao Zhan-Hu,Huang Shu-Shi. Analysis of components difference of yeast strains based on laser tweezers Raman spectroscopy combined with multistatistical analysis[J]. Spectroscopy and Spectral Analysis, 2011, 31(2): 412-417. DOI: 10.3964/j.issn.1000-0593(2011)02-0412-06 | |
| Authors: | Lai Jun-Zhuo Liu Bin Wang Gui-Wen Tao Zhan-Hu Huang Shu-Shi |
| Affiliation: | LAI Jun-zhuo1,2,LIU Bin3,WANG Gui-wen1,TAO Zhan-hua1,HUANG Shu-shi1 1.Lab of Biophysics,Guangxi Academy of Sciences,Nanning 530003,China 2.College of Life Sciences and Technology,Guangxi University,Nanning 530005,China 3.Institute of Applied Microbiology,Agricultural College of Guangxi University,China |
| Abstract: | The combination of laser tweezers and con-focusing Raman spectroscopy(LTRS) has made it possible to investigate single cells in aqueous media.In our experiments,a modified method of Percoll density gradient centrifugation was developed for isolating synchronous cells from six yeast strains and Raman spectra of single yeast cells were collected by LTRS as well,in which multiple statistical analysis,principal component analyses(PCA) and discriminant function analysis(DFA) were applied to distinguish synchrono... |
| Keywords: | Raman spectroscopy Multistatistical analysis Single cell Yeast strains |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |