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| 红外光谱用于大肠肿瘤诊断的初步研究 | |
| 作者姓名： | Yao HW Liu YQ Fu W Shi XY Zhang YF Xu YZ |
| 作者单位： | 1. 北京大学第三医院普通外科,北京,100191 2. 北京大学医学部病理学系,北京,100191 3. 北京大学化学与分子工程学院,北京,100871 |
| 基金项目： | 国家自然科学基金项目(50673005); 北京市优秀人才培养项目(2010D009001000005)资助 |
| 摘 要： | 探索红外光谱诊断大肠肿瘤性质的可行性。联合使用衰减全反射探头与傅里叶变换红外光谱仪测定新鲜离体大肠18个癌组织和10个良性腺瘤的红外光谱,将两组光谱的峰位及相对峰强等指标进行比较。与良性腺瘤相比,大肠癌的红外光谱具有如下特征:(1)与脂类相关的谱带2 925和1 740cm-1峰强比I2 925/I1 460(p=0.018)和I1 740/I1 460(p=0.009)明显降低,表明癌组织的脂类相对含量降低;(2)与蛋白质相关峰强比值I3 375/I1 460(p=0.012)和I1 550/I1 460(p=0.041)明显升高,表明蛋白质相对含量明显升高;(3)与核酸相关的1 080cm-1处峰位发生明显的蓝移(p=0.039),峰强比I1 080/I1 460明显升高(p=0.036),说明癌组织中核酸相对脂类含量明显增加;(4)谱带1 305cm-1处峰位发生明显的红移(p=0.041),其指认有待进一步研究。初步研究结果表明,傅里叶变换红外光谱分析可能成为一种快速鉴别大肠肿瘤性质的新方法。 |
| 关 键 词： | 傅里叶变换红外光谱 大肠肿瘤 诊断 |
Initial research on Fourier transform infrared spectroscopy for the diagnosis of colon neoplasms |
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| Yao HW,Liu YQ,Fu W,Shi XY,Zhang YF,Xu YZ.Initial research on Fourier transform infrared spectroscopy for the diagnosis of colon neoplasms[J].Spectroscopy and Spectral Analysis,2011,31(2):297-301. | |
| Authors: | Yao Hong-Wei Liu Ya-Qi Fu Wei Shi Xue-Ying Zhang Yuan-Fu Xu Yi-Zhuang |
| Institution: | YAO Hong-wei1,LIU Ya-qi1,FU Wei1,SHI Xue-ying2,ZHANG Yuan-fu3,XU Yi-zhuang3 1.Department of General Surgery,Peking University Third Hospital,Beijing 100191,China 2.Department of Pathology,Peking University Health Science Center,China 3.College of Chemistry and Molecular Engineering,Peking University,Beijing 100871,China |
| Abstract: | To explore the feasibility of Fourier transform infrared spectrometry(FTIR) for the diagnosis of colon neoplasms,fresh samples of eighteen cancers and ten adenomas were collected during colon surgery and were measured by FTIR via probe of attenuated total reflection(ATR).The peak position and the intensity of all bands were measured and compared between the malignant and benign groups.Results show that the FTIR of malignant neoplasm was different from that of benign one.(1)For the bands related to lipid: Th... |
| Keywords: | Fourier transform infrared spectrometry Colon neoplasm Diagnosis |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |