| 稻米色氨酸含量近红外反射光谱全局和局部飞速定标技术研究 |
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| 引用本文： | Zhang B,Zhang DP,Zhang WB,Wang KM,Li G,Yao YZ,Wu JG,Shi CH. 稻米色氨酸含量近红外反射光谱全局和局部飞速定标技术研究[J]. 光谱学与光谱分析, 2011, 31(1): 73-76. DOI: 10.3964/j.issn.1000-0593(2011)01-0073-04 |
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| 作者姓名： | Zhang B Zhang DP Zhang WB Wang KM Li G Yao YZ Wu JG Shi CH |
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| 作者单位： | 浙江大学农业与生物技术学院农学系,浙江杭州,310029 |
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| 基金项目： | 国家自然科学基金项目(30500319); 浙江省科技厅科技攻关项目(2007C12902); 浙江省自然科学基金项目(Y3080217)资助 |
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| 摘 要： | 色氨酸是人类一种必需氨基酸,也是稻米中一种重要的限制性氨基酸。从4年份1256份材料中选择出272份有代表性的样品,采用碱水解-分光光度法测定了其色氨酸含量。比较不同定标方法的预测结果发现,运用改良的偏最小二乘法(modified partial least square,MPLS)的全局(Global)定标方法和局部(Lo-cal)飞速定标方法的预测效果较佳,基于精米粉光谱建立的方程的预测标准误差均为0.007%,外部验证决定系数分别为87.1%和87.4%,可用于定量分析;而基于糙米光谱建立的定标方程的预测效果略差,但仍具有良好的预测能力。研究结果表明,近红外光谱技术可作为水稻育种中间材料的快速筛选和食品工业中稻米原料的品质监控手段。
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| 关 键 词： | 精米 色氨酸含量 近红外漫反射光谱 全局定标 局部飞速定标 |
Study on the global and local calibration methods of tryptophan content in rice by near infrared reflectance spectroscopy |
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| Zhang Bin,Zhang Da-Peng,Zhang Wen-Bin,Wang Ke-Min,Li Guan,Yao Ya-Zhen,Wu Jian-Guo,Shi Chun-Hai. Study on the global and local calibration methods of tryptophan content in rice by near infrared reflectance spectroscopy[J]. Spectroscopy and Spectral Analysis, 2011, 31(1): 73-76. DOI: 10.3964/j.issn.1000-0593(2011)01-0073-04 |
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| Authors: | Zhang Bin Zhang Da-Peng Zhang Wen-Bin Wang Ke-Min Li Guan Yao Ya-Zhen Wu Jian-Guo Shi Chun-Hai |
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| Affiliation: | ZHANG Bin,ZHANG Da-peng,ZHANG Wen-bin,WANG Ke-min,LI Guan,YAO Ya-zhen,WU Jian-guo*,SHI Chun-hai* Department of Agronomy,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310029,China |
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| Abstract: | Tryptophan is one of the essential amino acids in human,and also considered as an important limiting amino acid in rice.Alkali hydrolysis method was used to determine the tryptophan content of 272 representative samples selected from the 1 256 samples in four years.Based on the milled rice flour spectroscopy,by comparing the prediction abilities of the equations with global calibrations and local calibration,the global calibration method with MPLS and the local calibration method were better than others.The... |
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| Keywords: | Milled rice Tryptophan content Near infrared reflectance spectroscopy(NIRS) Global calibration Local calibration |
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