| 光柄筒冠花核糖体DNA ITS区序列 |
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| 引用本文： | 施苏华,文军.光柄筒冠花核糖体DNA ITS区序列[J].中山大学学报(自然科学版),1998,37(2):52-56. |
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| 作者姓名： | 施苏华 文军 |
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| 作者单位： | 中山大学生命科学学院 |
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| 基金项目： | 国家八五（85－910－11－01）课题基金,国家自然科学基金 |
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| 摘 要： | 测定中国特有植物光西风筒化的ＩＴＳ区序列，分析了其结构特征，Ｇ＋Ｃ百分含质量及序列变异性，并比较了光西风筒冠花与其他被子植物的ＩＴＳ区序列特征。光柄筒冠花的ＩＴＳ区和５．８Ｓ ＤＮＡ的总长度为６０９个核苷酸。其中ＩＴＳ－１为２０７ｂｐ，ＩＴＳ－２为２３６ｂｐ，５．８ＳｒＤＮＡ为１６６ｂｐ；总的ω（Ｇ＋Ｃ）为５９．１１％，其中ＩＴＳ－１为６４．２５％，ＩＴＳ－２为５７．６３％和５．８ＳｒＤＮＡ为５３
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| 关 键 词： | 光柄筒冠花 ITS区 核糖体 DNA 唇形科 |
Sequence Analyses of ITS Regions of nrDNA from Siphocranion nudipes Kudo (Lamiaceae) |
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| Shi Suhua,Wen Jun,Zhu Lingfeng,\ Zhu Zhongbao,Huang Lingying.Sequence Analyses of ITS Regions of nrDNA from Siphocranion nudipes Kudo (Lamiaceae)[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,1998,37(2):52-56. |
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| Authors: | Shi Suhua Wen Jun Zhu Lingfeng \ Zhu Zhongbao Huang Lingying |
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| Institution: | Shi Suhua 1) Wen Jun 2) Zhu Lingfeng 3) \ Zhu Zhongbao 3) Huang Lingying 3) |
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| Abstract: | The sequence of internal transcribed spacer(ITS) regions of nuclear ribosomal DNA (nrDNA) was determined for the endemic species Siphocranion nudipes (Hemsl ) Kudo (Lamiaceae) from China. This is the first sequence report of the ITS regions for Lamiaceae. The entire ITS and 5 8S regions of Siphocranion nudipes are 609 bases long with an ITS 1 of 207 bp, an ITS 2 of 236 bp and a 5 8S of 166 bp. The overall G C content was 59 11% with 64 25% in ITS 1, 57 63% in ITS 2, and 53 61% in 5 8S.The numerous copies of the ITS sequences within an individual in Siphocranion nudipes showed a high level of sequence homogeneity and only three nucleotide positions were ambiguous. This sequence will serve as a basis for determining the phylogenetic status of Siphocranion nudipes within Lamiaceae and for indepth studies on the conservation of this endemic species from subtropical areas of China. |
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| Keywords: | sequence Siphocranion nudipes internal transcribed spacer regions |
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