基于氧化铒-石墨烯氧化物复合纳米材料的葡萄糖氧化酶直接电化学性能及对葡萄糖的检测

将稀土纳米材料Er₂O₃用于构建葡萄糖生物传感器。Er₂O₃和氧化石墨烯形成复合基底，将葡萄糖氧化酶（GOD）固载在玻碳电极表面。首先利用SEM和XRD技术对所制备的Er₂O₃和氧化石墨烯纳米材料进行表征。利用EIS和CV对整个生物传感器制备过程进行表征。Er₂O₃的存在能有效的保持GOD的生物活性并加速其电子传递速率。由于Er₂O₃和氧化石墨烯之间的协同效应，使得制备的传感器具有一对良好的氧化还原峰，证实GOD和电极之间的直接传递性能。当用于对葡萄糖的电催化氧化时，传感器的CV响应随着葡萄糖浓度的增加而变弱。在葡萄糖浓度为1~10 mmol·L^-1范围内，CV响应值与葡萄糖浓度成线性关系。此外，传感器具有好的稳定性和重现性。

Er2O3-Graphene Oxide Nanocomposite Supported Glucose Oxidase: Direct Electrochemistry and Biosensing to Glucose

A new rare earth oxide of Er₂O₃ was employed for the construction of glucose biosensor.Er₂O₃ was mixed with graphene oxide (GO) to form the supported matrix for immobilization of glucose oxidase (GOD) onto the glassy carbon electrode (GCE).The nanomaterials of Er₂O₃ and GO were firstly synthesized and characterized by SEM,XRD.The fabrication process for the biosensor was monitored by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV).The presence of Er₂O₃ could effectively maintain the bioactivity of GOD and enhance the electron transfer rate.The prepared biosensor showed a pair of distinct and well-defined redox peaks,indicating the fast direct electron transfer (DET) rate between the redox-active site of GOD and GCE,which could be attributed to the synergistic effect of the GO/Er₂O₃ nanocomposite.When employed to the electrocatalytic detection of glucose,the CV response of the prepared biosensor decreased against the concentrations of glucose.The calibration curve corresponding to the CV response was linear against the concentrations of glucose ranging from 1 to 10 mmol·L^-1.Moreover,the biosensor showed good stability and reproducibility.