Liquid membrane transport of cytochrome c using a calix[6]arene carboxylic acid derivative as a carrier |
| |
Authors: | Tatsuya Oshima Akinori Suetsugu Yoshinari Baba Yuka Shikaze Keisuke Ohto Katsutoshi Inoue |
| |
Institution: | 1. Department of Applied Chemistry, Faculty of Engineering, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan;2. Department of Applied Chemistry, Faculty of Science and Engineering, Saga University, 1-Honjo, Saga 840-8502, Japan |
| |
Abstract: | The present study examined the liquid membrane transport of the cationic protein cytochrome c, using the macrocyclic compound calix6]arene, which is a carboxylic acid derivative, as a carrier. The transport rate was governed by carrier concentration and the pH gradient between the feed and the receiving phases, as well as the salt concentration in the aqueous phases. Transport of cytochrome c was examined using a series of calixn]arene carboxylic acid derivatives (n = 4, 6 and 8). Cytochrome c successfully permeated membranes in the presence of the calix6]arene derivative. Liquid membrane separation of cytochrome c from a mixture of cationic proteins was demonstrated under optimal conditions. Cytochrome c was selectively extracted by the calix6]arene carboxylic acid derivative and 77% of the extracted cytochrome c was recovered into the receiving phase. In this liquid membrane system, which discriminates between the number of lysine residues on the surface of proteins, cationic proteins with similar molecular weights and pIs were separated with macrocyclic compounds. |
| |
Keywords: | Liquid membrane Calixarene Protein Cytochrome c Facilitated transport |
本文献已被 ScienceDirect 等数据库收录! |
|