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Isolation and Characterization of Adipose-derived Mesenchymal Stem Cells (ADSCs) from Cattle |
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| Authors: | Taofeng Lu Hui Xiong Kunfu Wang Shuo Wang Yuehui Ma Weijun Guan |
| Institution: | 1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China 2. State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150001, China 3. Medical College, Jiamusi University, Jiamusi, 154007, China |
| Abstract: | Adipose-derived mesenchymal stem cells (ADSCs) were isolated from the adult adipose tissue of 2-year-old cattle, and then characterized by immunofluorescence and RT-PCR. We found that primary bADSCs could be expanded for 25 passages. Expression of β-integrin, CD44, and CD73 was observed by immunofluorescence and RT-PCR. Passage 3 bADSCs were successfully induced to differentiate into osteoblasts and adipocytes. The results indicate the potential for multi-lineage differentiation of bADSCs that may represent an ideal candidate for cellular transplantation therapy. |
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