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EFFECT OF SPHERICAL ABERRATION INTRODUCED BY WATER SOLUTION ON TRAPPING FORCE
Authors:Yao Xin-cheng  Li Zhao-lin  Guo Hong-lian  Cheng Bing-ying  Han Xue-hai  Zhang Dao-zhong
Affiliation:Optical Physics Laboratory, Institute of Physics and Center for Condensed Matter Physics, Chinese Academy of Sciences, Beijing 100080, China; State Key Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
Abstract:Trapping force of an optical tweezers system with an oil immersion objective is calculated with a ray-optics model. Results indicate that the trapping force will be decreased as a result of the introduction of spherical aberration, which is caused by the refractive mismatch between objective oil and water, when the sample manipulated is suspended in a water solution. The effect of spherical aberration will be serious when the detection depth of the optical tweezers is enhanced.
Keywords:spherical aberration   optical tweezers   trapping force
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