Solid-phase extraction of midazolam and two of its metabolites from plasma for high-performance liquid chromatographic analysis. |
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| Authors: | V Sautou J Chopineau M P Terrisse P Bastide |
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| Institution: | Laboratoire de Pharmacologie et Pharmacie Clinique et Biotechniques, U.F.R. Pharmacie, Clermont-Ferrand, France. |
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| Abstract: | A rapid, sensitive and selective assay of midazolam and two of its metabolites in plasma, based on high-performance liquid chromatography, has been developed. The compounds are subjected to solid-phase extraction, using C18 cartridges (Bond-Elut). Recoveries are in excess of 90% for midazolam and its metabolites. The limit of quantitation of the assay is 50 ng/ml of plasma for each compound. |
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