| 壳聚糖固定化总状毛霉MR137-3蛋白酶的性质 |
| |
| 引用本文： | 张勇侠,黄新河,姜骅,雷芳,李佳,刘克武.壳聚糖固定化总状毛霉MR137-3蛋白酶的性质[J].化学研究与应用,2004,16(4):472-474. |
| |
| 作者姓名： | 张勇侠 黄新河 姜骅 雷芳 李佳 刘克武 |
| |
| 作者单位： | 四川大学生命科学学院,四川,成都,610064;四川大学生命科学学院,四川,成都,610064;四川大学生命科学学院,四川,成都,610064;四川大学生命科学学院,四川,成都,610064;四川大学生命科学学院,四川,成都,610064;四川大学生命科学学院,四川,成都,610064 |
| |
| 摘 要： | 以壳聚糖为载体，戊二醛作交联剂，将总状毛霉MR137—3蛋白酶固定在壳聚糖上。研究了戊二醛浓度，给酶量，处理时间对MR137—3蛋白酶固定化的影响。同时对固定化酶与游离酶的热稳定性、最适pH、最适温度以及脲、有机溶剂、金属离子的影响等理化性质进行了探讨。
|
| 关 键 词： | 总状毛霉MR137-3 壳聚糖 戊二醛 固定化 |
| 文章编号： | 1004-1656(2004)04-0472-03 |
| 修稿时间： | 2003年10月22 |
Study on the method of immobolizing mucor racemosus137-3 proteinase with chitosan |
| |
| ZHANG Yong-xia,HUANG Xin-he,LIU Ke-wu.Study on the method of immobolizing mucor racemosus137-3 proteinase with chitosan[J].Chemical Research and Application,2004,16(4):472-474. |
| |
| Authors: | ZHANG Yong-xia HUANG Xin-he LIU Ke-wu |
| |
| Abstract: | This paper deals with the immobilization of mucor racemosus137-3 proteinase on chitosan(as a support) by glutaraldehyde(as a cross-linking reagent).The best conditions of immobilization,such as the weights of wet chitosan and proteinase,the treating time by glutaraldehyde,and the concentration of glutaraldehyde were studied. And some characters of immobilized and free mucor racemosus137-3 proteinase, such as optimum temperature, optimum pH , stabilities to heat and the effect of metal ionic, urea, and organic solvents were studied and compared. |
| |
| Keywords: | mucor racemosus137-3 chitosan glutaraldehyde immobilization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
