NMR study of tomato pericarp tissue by spin-spin relaxation and water self-diffusion |
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Authors: | F P Duval M Cambert F Mariette |
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Institution: | 1. Cemagref, UR Technologie des Equipements Agroalimentaires, CS 64426, 17 Avenue de Cucillé, 35044, Rennes, Cedex, France
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Abstract: | Pericarp tissues of tomato varieties Quest and Cameron were studied by low-field nuclear magnetic resonance (NMR) at a controlled temperature of 20°C. The spin-spin relaxation times and the water diffusion coefficients were measured with Carr-Parcell-Meiboom-Gill and pulsed field gradient multi-spin-echo (PFGMSE) NMR sequences. Four relaxing components were extracted from the spin-spin relaxation. The components withT 2=11 ms,T 2=65 ms,T 2=430 ms andT 2=1500 ms were related to the nonexchangeable protons and water proton in each cell compartment (i.e., cell wall-extracellular space, cytoplasm and vacuole, respectively). In contrast to the relative intensities, theT 2 values appeared insensitive to variety and harvest period. The difference in relative intensity was related to the size of the pericarp cell. The water self-diffusion coefficients for each cell compartment were determined simultaneously with the PFGMSE sequence. The water self-diffusion coefficients for the vacuole and cytoplasm were not affected by the harvest date or variety. However, the water self-diffusion in the cell wall-extracellular space was significantly different between the two varieties. |
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