Spatially resolved (semi)quantitative determination of iron (Fe) in plants by means of synchrotron micro X-ray fluorescence |
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Authors: | Roberto Terzano Matthias Alfeld Koen Janssens Bart Vekemans Tom Schoonjans Laszlo Vincze Nicola Tomasi Roberto Pinton Stefano Cesco |
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Institution: | 1. Department of Soil, Plant and Food Sciences, University of Bari “Aldo Moro”, Via Amendola 165/A, 70126, Bari, Italy 2. Department of Chemistry, University of Antwerp, Groenenborgerlaan 171, 2020, Antwerp, Belgium 3. Department of Analytical Chemistry, Ghent University, Krijgslaan 281, 9000, Ghent, Belgium 4. Department of Agricultural and Environmental Sciences, University of Udine, Via delle Scienze 206, 33100, Udine, Italy 5. Faculty of Science and Technology, Free University of Bozen/Bolzano, Piazza Università 5, 39100, Bolzano, Italy
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Abstract: | Iron (Fe) is an essential element for plant growth and development; hence determining Fe distribution and concentration inside plant organs at the microscopic level is of great relevance to better understand its metabolism and bioavailability through the food chain. Among the available microanalytical techniques, synchrotron μ-XRF methods can provide a powerful and versatile array of analytical tools to study Fe distribution within plant samples. In the last years, the implementation of new algorithms and detection technologies has opened the way to more accurate (semi)quantitative analyses of complex matrices like plant materials. In this paper, for the first time the distribution of Fe within tomato roots has been imaged and quantified by means of confocal μ-XRF and exploiting a recently developed fundamental parameter-based algorithm. With this approach, Fe concentrations ranging from few hundreds of ppb to several hundreds of ppm can be determined at the microscopic level without cutting sections. Furthermore, Fe (semi)quantitative distribution maps were obtained for the first time by using two opposing detectors to collect simultaneously the XRF radiation emerging from both sides of an intact cucumber leaf. Figure Elemental distribution maps within intact tomato roots as determined by confocal micro X‐ray fluorescence |
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