Dependency of Cellular Decision Making in Physiology and Influence of Preceding Growth Conditions

Events from the past growth conditions influence the course of physiology, and it gets reflected in the present cell behaviour. During this process, cells acquire a metabolic option which carries signature of the past, and it dictates the performance in the present situation. Study uses Escherichia coli as a sample organism wherein three scenarios of preceding growth conditions were created with varying nutritional status and pre-treatment strategies. This exercise leads to different seed cultures, which were subjected to growth using the four different substrates. The different seed culture behaviours were analysed by observing the respirometric rate of the seed culture and were followed by growth dynamics with different substrates. These two data sets were independently analysed by three-way ANOVA to arrive at strategic coupling of programming conditions to relate the available (respirometric rates) and executable physiology (growth kinetics).