Fishing of α-Glucosidase’s Ligands from Aloe vera by α-Glucosidase Functionalized Magnetic Nanoparticles |
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| Authors: | Hao Yuan Hong Wan Yi-Kao Hu Emmanuel Ayodeji Ayeni Qiang Chang Chao Ma Xun Liao |
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| Institution: | 1.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; (H.Y.); (H.W.); (Y.-k.H.); (E.A.A.); (Q.C.);2.University of Chinese Academy of Sciences, Beijing 100049, China;3.Phytochemistry Laboratory, Tibet Plateau Institute of Biology, Lhasa 850001, China |
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| Abstract: | α-Glucosidase was immobilized on magnetic nanoparticles (MNPs) for selective solid-phase extraction of the enzyme’s ligands present in Aloe vera, which is a medicinal plant used for the treatment of various diseases and possesses anti-diabetic activity. One new compound, aloeacone (2), together with two known compounds, aloenin aglycone (1) and aloin A (3), were fished out as the enzyme’s ligands. The structure of 2 was determined by HR-MS and comprehensive NMR techniques. Compound 3 exhibited a weak inhibitory effect on α-glucosidase, while compounds 1 and 2 were found to possess activation effects on the enzyme for the first time. It is interesting that both an inhibitor and agonists of α-glucosidase were fished out in one experiment. |
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| Keywords: | Aloe vera α -glucosidase ligand fishing magnetic nanoparticles |
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