Alpha-Glucosidase Inhibitory Assay-Screened Isolation and Molecular Docking Model from Bauhinia pulla Active Compounds |
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| Authors: | Sukanya Dej-adisai Ichwan Ridwan Rais Chatchai Wattanapiromsakul Thanet Pitakbut |
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| Institution: | 1.Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai 90112, Songkhla, Thailand; (I.R.R.); (C.W.);2.Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta 55164, Indonesia;3.Department of Biochemical and Chemical Engineering, Technical University of Dortmund, 44227 Dortmund, Germany; |
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| Abstract: | The aim of this research was to establish the constituents of Bauhinia pulla as anti-diabetic agents. A phytochemistry analysis was conducted by chromatographic and spectroscopic techniques. The alpha-glucosidase inhibitory assay screening resulted in the isolation of eight known compounds of quercetin, quercitrin, luteolin, 5-deoxyluteolin, 4-methyl ether isoliquiritigenin, 3,2′,4′-trihydroxy-4-methoxychalcone, stigmasterol and β-sitosterol. Ethanol leaf extracts showed potential effects, which led to a strong inhibitory activity of isolated quercetin at 138.95 µg/mL and 5.41 µg/mL of IC50, respectively. The docking confirmed that flavonoids and chalcones had the same potential binding sites and responsibilities for their activity. This study was the first report of Bauhinia pulla chemical constituents and its alpha-glucosidase inhibition. |
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| Keywords: | Bauhinia pulla anti-diabetic alpha-glucosidase inhibition molecular docking |
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