HPTLC and ATR/FTIR Characterization of Antioxidants in Different Rosemary Extracts |
| |
| Authors: | Snezana Agatonovic-Kustrin Ksenia S Balyklova Vladimir Gegechkori David W Morton |
| |
| Institution: | 1.Department of Pharmaceutical and Toxicological Chemistry named after Arzamastsev of the Institute of Pharmacy, I.M. Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, Russia; (K.S.B.); (V.G.); (D.W.M.);2.School of Pharmacy and Biomedical Sciences, La Trobe Institute for Molecular Sciences, La Trobe University, Edwards Rd, Bendigo 3550, Australia |
| |
| Abstract: | The effect of spontaneous fermentation by lactic acid bacteria on the extraction yield of bioactive compounds and antioxidant activity from rosemary leaf extracts was investigated using high-performance thin-layer chromatography (HPTLC). Brining and spontaneous fermentation with lactic acid bacteria more than doubled extraction of polyphenolics and antioxidants from the rosemary leaves. The results show that lactic acid fermentation enhances antioxidant activity in extracts by increasing the total phenolic content but does not increase extraction of phytosterols. Increased extraction of phenolic oxidants during fermentation assisted extraction, results from the in situ generated natural eutectic solvent from the plant sample. ATR-FTIR spectra from the bioactive bands suggests that this increased antioxidant activity is associated with increased extraction of rosmarinic acid, depolymerised lignin, abietane diterpenoids and 15-hydroxy-7-oxodehydroabietic acid. |
| |
| Keywords: | rosemary antioxidants HPTLC fingerprints fermentation assisted extraction fractional freezing abietic acid natural eutectic solvent |
|
|
