Immunomodulatory Activity of Carboxymethyl Pachymaran on Immunosuppressed Mice Induced by Cyclophosphamide |
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| Authors: | Feng Liu Lijia Zhang Xi Feng Salam A Ibrahim Wen Huang Ying Liu |
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| Institution: | 1.College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China; (F.L.); (L.Z.); (W.H.);2.Department of Nutrition, Food Science and Packaging, San Jose State University, San Jose, CA 95192, USA;3.Department of Family and Consumer Sciences, North Carolina A&T State University, 171 Carver Hall, Greensboro, NC 27411, USA; |
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| Abstract: | The effects of immunomodulatory activity of two types of carboxymethyl pachymaran (CMP-1 and CMP-2) on cyclophosphamide (CTX)-induced mice were investigated. Both CMP-1 and CMP-2 were found to restore the splenomegaly and alleviate the spleen lesions and the mRNA expressions of TLR4, MyD88, p65 and NF-κB in spleen were also increased. CMP-1 and CMP-2 could enhance the immunity by increasing the levels of TNF-α, IL-2, IL-6, IFN-γ, Ig-A and Ig-G in serum. In addition, CMP-1 could increase the relative abundance of Bacteroidetes and reduce the relative richness of Firmicutes at the phylum level. CMP-1 and CMP-2 could reduce the relative abundance Erysipelatoclostridum at the genus level. CMP-1 and CMP-2 might enhance the immune function of immunosuppression mice by regulating the gene expression in the TLR4/NF-κB signaling pathway and changing the composition and abundance of the intestinal microbiota. The results suggested that CMP-1 and CMP-2 would be as potential immunomodulatory agents in functional foods. |
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| Keywords: | Poria cocos carboxymethyl pachymaran immunomodulatory cyclophosphamide gut microbiota |
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