A Galactosidase-Activatable Fluorescent Probe for Detection of Bacteria Based on BODIPY |
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| Authors: | Xi Chen Yu-Cong Liu Jing-Jing Cui Fang-Ying Wu Qiang Xiao |
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| Institution: | 1.College of Chemistry, Nanchang University, Nanchang 330031, China;2.Key Laboratory of Organic Chemistry in Jiangxi Province, Institute of Organic Chemistry, Jiangxi Science & Technology Normal University, Nanchang 330013, China; (Y.-C.L.); (J.-J.C.) |
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| Abstract: | Pathogenic E. coli infection is one of the most widespread foodborne diseases, so the development of sensitive, reliable and easy operating detection tests is a key issue for food safety. Identifying bacteria with a fluorescent medium is more sensitive and faster than using chromogenic media. This study designed and synthesized a β-galactosidase-activatable fluorescent probe BOD-Gal for the sensitive detection of E. coli. It employed a biocompatible and photostable 4,4-difluoro-3a,4a-diaza-s-indancene (BODIPY) as the fluorophore to form a β-O-glycosidic bond with galactose, allowing the BOD-Gal to show significant on-off fluorescent signals for in vitro and in vivo bacterial detection. This work shows the potential for the use of a BODIPY based enzyme substrate for pathogen detection. |
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| Keywords: | BODIPY β -galactosidase activity PET fluorescent |
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