Metallomics: An integrated biometal science |
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Authors: | RuiGuang Ge HongZhe Sun |
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Institution: | (1) Dept of Surgery, McGill University, Montreal, Quebec, Canada;(2) Montreal Proteomics Network, McGill University, Montreal, Quebec, Canada;(3) Dept of Medicine, McGill University, Montreal, Quebec, Canada;(4) Dept of Anatomy, McGill University, Montreal, Quebec, Canada |
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Abstract: | Metallomics is an emerging scientific area integrating the research fields related to the understanding of the molecular mechanisms
of metal-associated life processes and the entirety of metal and metalloid species within a cell or tissue type. In metallomics,
metalloproteins, metalloenzymes and other metal-containing biomolecules in a biological system are referred to as metallomes,
similar to genomes and proteomes in genomics and proteomics, respectively. This review discusses the concept of metallomics
with a focus on analytical techniques and methods, particularly the so-called hyphenated techniques which combine a high-resolution
separation technique (gel electrophoresis/laser ablation, chromatography or capillary electrophoresis) with a highly sensitive
detection method such as elemental (inductively coupled plasma, ICP) or molecular (electron spray ionization (ESI) or matrix-assisted
laser desorption/ionization (MALDI)) mass spectrometry, or nuclear X-ray fluorescence/absorption spectrometry. The applications
of these advanced analytical methods in the identification of metallo-/phospho-/seleno-proteins, probing of relationships
between structure and function of metalloproteins, and study of clinically used metallodrugs will be selectively outlined,
along with their advantages and limitations. |
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