The effect of an organic eluent modifier and pH on the separation of wheat-storage proteins: Application to the purification of γ-gliadins ofTriticum monococcum L |
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| Authors: | W. H. Vensel D. Lafiandra D. D. Kasarda |
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| Affiliation: | (1) US Department of Agriculture, Agricultural Research Service, Western Regional Research Center, 94710 Albany, CA, USA;(2) Department of Agrobiology and Agrochemistry, University of Tuscia, Via S. Camillo De Lellis, I-01100 Viterbo, Italy |
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| Abstract: | Summary Reversed-phase, high-pressure liquid chromatography has been used to separate similar protein (γ-gliadin) components from 70% ethanol extracts of endosperm flour from two different accessions of the diploid wheatTriticum monococcum L. The effect of acetonitrile as the organic eluent was compared to acetonitrile: 2-propanol (3:1) at two different pH's. Conditions for maximum resolution of the γ-gliadin components were found to be at pH 7.2 with acetonitrile: 2-propanol (3:1) as the eluent. These conditions allowed the components to be obtained in sufficient purity for further charaterization. The mention of firm names or trade products does not imply that they are endorsed or recommended by the US Department of Agriculture over other firms or similar products not mentioned. |
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| Keywords: | Column liquid chromatography Wheat proteins γ -gliadins Effect of pH on separation Effect of organic modifier on separation |
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