AUTOINDUCTION AND ALDEHYDE CHAIN-LENGTH EFFECTS ON THE BIOLUMINESCENT EMISSION FROM THE YELLOW PROTEIN ASSOCIATED WITH LUCIFERASE IN Vibrio fischeri STRAIN Y-1b |
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Authors: | Ki Woong CHO† Pio Colepicolo J Woodland Hastings |
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Institution: | Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138, USA |
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Abstract: | Abstract— A new strain of the yellow-light emitting bacterium Vibrio fischeri Y-lb, possessing a higher fraction of its emission in the yellow, was isolated and characterized. The in vivo yellow to blue ratio (Y/B) increases during growth in parallel with the autoinduction of luciferase, reaching a value above 5 at 17°C. This Y/B increase is attributed to concomitant increases in the intracellular levels of both luciferase and the specific protein associated with yellow emission, the yellow fluorescent protein (YFP), and to the association of the two proteins at higher concentrations. The yellow emission is rapidly but reversibly lost by brief exposures to a temperature of 27°C (5 s), and irreversibly so at 40°C, attributed to dissociation of the two proteins. A concentrated crude extract emits almost exclusively yellow light in the in vitro reaction, and theY/B ratio decreases with dilution. The Y/B ratio in vitro is also dependent on the aldehyde chain length; experiments with aldehyde analogs suggest that the aldehyde binding site of luciferase must be occupied for YFP to stimulate light emission. |
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