Molecular luminescence imaging |
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Authors: | A Roda M Guardigli M Mirasoli M Musiani |
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Institution: | a Department of Pharmaceutical Sciences, University of Bologna, via Belmeloro 6, 40126 Bologna, Italy b Laboratoire de Chimie Moléculaire, ECPM, 25 rue Becquerel, 67087 Strasbourg Cedex 02, France c Department of Experimental and Clinical Medicine, University of Bologna, via Massarenti 9, 40138 Bologna, Italy |
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Abstract: | Imaging techniques relying on different luminescence processes are powerful bioanalytical tools for life sciences. They are used in multiplexed quantitative assays in different analytical formats and to assess the spatial distribution of a given target molecule, chemical or biochemical process in macro- and microsamples, including the in vivo evaluation of biological and pathological processes. Here, recent progresses in luminescence imaging techniques are described, including new labels for fluorescence, time-resolved fluorescence and bio-chemiluminescence, multiplexed imaging microscopy techniques and whole-body luminescence imaging in live animals. |
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Keywords: | Bioluminescence Chemiluminescence Fluorescence Imaging Immunohistochemistry In situ hybridization Reporter gene Time-resolved fluorescence Whole-body imaging |
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