An EPR and DFT study on the primary radical formed in hydroxylation reactions of 2,6-dimethoxy-1,4-benzoquinone |
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Authors: | Haleh H Haeri Ivan Bogeski Rubin Gulaboski Valentin Mirceski Markus Hoth |
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Institution: | 1. Department of Biophysics, School of Medicine, Saarland University, Homburg, Germany;2. Institute for Physical Chemistry, Department of Chemistry, Martin-Luther-University, Halle (Saale), Germanyhaleh.hashemi-haeri@chemie.uni-halle.de;4. Faculty of Medical Sciences, Goce Del?ev University, Stip, Republic of Macedonia;5. Faculty of Natural Sciences and Mathematics, Department of Chemistry, “Ss Cyril and Methodius” University, Skopje, Republic of Macedonia |
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Abstract: | The quinone compound 2,6-dimethoxy-1,4-benzoquinone is hydroxylated in alkaline aqueous solution with pH above 12. Electron paramagnetic resonance experiments showed that two transient radicals are formed in this reaction. The radical appearing first is assigned to a one electron reduced 2,6-dimethoxy-1,4-benzoquinone, receiving the electron from an intermediate anionic hydroxylated species. For this primary radical, all proton couplings were determined (quinoid ring protons: 1.453?G, methyl protons: 0.795?G). The density functional theory method was applied to obtain electronic and structural information of the primary radical and a solution structure is suggested. For approaching the experimental hyperfine couplings in theoretical models, it was necessary to consider effects of external polarisation arising from water molecules near one carbonyl group, and the orientation of methoxy groups towards the quinone ring. With this approach, the secondary radical formed in the hydroxylation reaction, and the transient radicals found for other biologically important quinones (including coenzymes Q) and their hydroxylated species may become accessible. |
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Keywords: | Methoxy benzoquinones semi-quinone radical density functional theory reactive oxygen species metal ligands |
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