Electrophoretic determination of biogenic amines in biological fluids

The capabilities of the electrophoretic separation of biogenic amines (adrenalin, noradrenalin, dopamine, serotonin, metanephrine, and normetanephrine) under conditions of capillary zone electrophoresis and micellar electrokinetic chromatography with the introduction of complex-forming agents (18-crown-6, 4,13-diaza-18-crown-6, and sodium dodecyl sulfate as an ion-pair reagent) and acetonitrile as the constituents of a working electrolyte were demonstrated. A technique for the sampling of biological fluids (urine, blood plasma, and serum) with the use of solid-phase extraction on aluminum oxide and a C18 reversed-phase sorbent was developed. The capabilities of various versions of the preconcentration of biogenic amines were determined, which allowed us to decrease the limits of detection by a factor of hundreds.