Ultra-high pressure LC determination of glucosamine in shrimp by-products and migration tests of chitosan films |
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Authors: | Sanches-Silva Ana Ribeiro Tiago Albuquerque Tânia G Paseiro Perfecto Sendón Raquel de Quirós Ana Bernaldo López-Cervantes Jaime Sánchez-Machado Dalia I Valdez Herlinda Soto Angulo Inmaculada Aurrekoetxea Goizane P Costa Helena S |
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Affiliation: | Food and Nutrition Department, National Institute of Health Dr Ricardo Jorge, Lisbon, Portugal. anateress@gmail.com |
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Abstract: | Chitosan, a multiple applications molecule, was isolated from shrimp by-products by fermentation. The amount of chitosan in the solid fraction of the fermented extract was measured after its conversion in the respective glucosamine units. The procedure includes an acid hydrolysis (110 °C, 4 h with HCl 8 M) and a derivatization with 9-fluorenylmethyl chloroformate (Fmoc-Cl). Ultra-high-pressure liquid chromatography method was developed and optimized. Excellent peaks resolution was achieved in just 10 min. The method was evaluated in what concerns to validation parameters such as linearity, repeatability, quantification limit, and recovery. Migration tests of films prepared with chitosan were carried out in two simulants: ultrapure water and ethanol 95% (v/v). |
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Keywords: | Chitosan / Glucosamine / Migration tests / Shrimp by‐products / Ultra‐high‐pressure liquid chromatography |
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