Intracellular pH measurements made simple by fluorescent protein probes and the phasor approach to fluorescence lifetime imaging |
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| Authors: | Battisti Antonella Digman Michelle A Gratton Enrico Storti Barbara Beltram Fabio Bizzarri Ranieri |
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| Affiliation: | NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, Piazza San Silvestro 12, 56127 Pisa, Italy. antonella.battisti@sns.it r.bizzarri@sns.it |
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| Abstract: | A versatile pH-dependent fluorescent protein was applied to intracellular pH measurements by means of the phasor approach to fluorescence lifetime imaging. By this fit-less method we obtain intracellular pH maps under resting or altered physiological conditions by single-photon confocal or two-photon microscopy. |
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