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Bioanalytical methods applied to endocrine disrupting polychlorinated biphenyls, polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans. A review |
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| Authors: | J. Dí az-Ferrero,M.C. Rodrí guez-Larena,L. Comellas,B. Jimé nez |
| Affiliation: | Environmental Laboratory, C.E.T.S. Institut Químic de Sarrià, Universitat Ramon Llull, Via Augusta 390, 08017, Barcelona, Spain Section of Chromatography, Department of Analytical Chemistry, C.E.T.S. Institut Químic de Sarrià, Universitat Ramon Llull, Via Augusta 390, 08017, Barcelona, Spain Department of Instrumental Analysis and Environmental Chemistry, Institute of Organic Chemistry, C.S.I.C., Juan de la Cierva 3, 28006, Madrid, Spain |
| Abstract: | The usual methods for determining polychlorinated dibenzo-p-dioxins (PCDD), polychlorinated dibenzofurans (PCDF) and polychlorinated biphenyls (PCB) are generally expensive and time consuming. This fact has favored the development of faster and cheaper techniques, based on immunoassays and bioassays. This paper reviews these bioanalytical methods and their analytical importance at the present moment. |
| Keywords: | Abbreviations: AHH, aryl hydrocarbon hydroxylase CALUX, chemical-activated luciferase expression CEH, chicken embryo hepatocytes DMSO, dimethyl sulfoxide EIA, enzyme immunoassay ELISA, enzyme-linked immunosorbent assay EROD, 7-ethoxyresorufin O-deethylase HAP, hydroxylapatite HRGC-HRMS, high resolution gas chromatography-high resolution mass spectrometry HRP, horseradish peroxidase PBB, polybrominated biphenyl PCB, polychlorinated biphenyl PCDD, polychlorinated dibenzo-p-dioxin PCDF, polychlorinated dibenzofuran RIA, radioimmunoassay TCDD, tetrachlorodibenzo-p-dioxin TEF, toxic equivalency factor TEQ, toxic equivalent |
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