| Cross-talk correction in dual-labeled fluorescent microarray scanning |
| |
| 作者姓名： | 王立强 陆祖康 倪旭翔 |
| |
| 作者单位： | State Key Lab of Modern Optical Instrumentation，Zhejiang University，Hangzhou 310027，State Key Lab of Modern Optical Instrumentation，Zhejiang University，Hangzhou 310027，State Key Lab of Modern Optical Instrumentation，Zhejiang University，Hangzhou 310027 |
| |
| 基金项目： | This project was supported by the Key Program of National Natural Science Foundation of China under Grant No.30227002. |
| |
| 摘 要： | Cross-talk phenomenon in dual-labeled fluorescent microarray scanning is analyzed from cross-excitation and cross-emission. It is turned out that the spectral overlap of the fluorophores is crucial for cross-talk error, and this error can be corrected by an image subtraction method. The experiment was successfully applied to separate the Cy3 channel and the Cy5 channel in microarray scanning. The cross-talk error was reduced from more than 1% to about 0.1%.
|
| 本文献已被 CNKI 等数据库收录！ |
