Enrichment of the spindle fiber attachments from mouse liver cell nuclei |
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| Authors: | L J Shi Z M Ni S F Lu G Wang H L Qi Q X Su |
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| Affiliation: | Shanghai Institute of Cell Biology, Academia Sinica. |
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| Abstract: | Spindle fiber attachments (SFAs) were enriched in a fraction of nuclei isolated from mouse livers. The enrichment method combines sonication, treatment with 2 mol/L NaCl and high speed centrifugation. SFA was enriched 27-fold on the average (n = 4) when measured by radioimmunoassay. The basic method offers opportunities for further increases of yield and for the enrichment of SFA of other vertebrates. |
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