Detection and relative quantification of proteins by surface enhanced Raman using isotopic labels |
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Authors: | Deb Shirshendu K Davis Brandon Knudsen Giselle M Gudihal Ravindra Ben-Amotz Dor Davisson V Jo |
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Institution: | Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907, USA. |
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Abstract: | Accurate quantification of protein content and composition has been achieved using isotope-edited surface enhanced resonance Raman spectroscopy. Synthesis of isotopomeric Rhodamine dye-linked bioconjugation reagents enabled direct labeling of surface lysines on a variety of proteins. When separated in polyacrylamide gels and stained with silver nanoparticles. The spectral signatures reflect the expected statistical distribution of isotopomeric labels on the labeled proteins in the gel matrix format without interference from protein features. |
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