Sub-second isoelectric focusing in free flow using a microfluidic device |
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| Authors: | Xu Yi Zhang Chao-Xuan Janasek Dirk Manz Andreas |
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| Affiliation: | Imperial College London, Chemistry Department, Exhibition Road, London, UK SW7 2AZ. |
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| Abstract: | Using a microfabricated chip with a bed volume of 0.2 microL we demonstrate the validity of the scaling laws for molecular mass transport of isoelectric focusing (IEF) in free flow. Nano- or microlitre sample volumes can be concentrated within 430 ms by a factor of up to 400. These very fast performances make the chip applicable to proteomic analysis and for continuous monitoring of biochemical processes. |
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