| 黄芩素纯度标准物质的定值及其不确定度评定 |
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| 引用本文： | 姚换方,全灿,刘军,侯彩霞.黄芩素纯度标准物质的定值及其不确定度评定[J].化学分析计量,2012,21(6):4-8. |
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| 作者姓名： | 姚换方 全灿 刘军 侯彩霞 |
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| 作者单位： | 1. 河北联合大学化学工程学院,河北唐山,063009
2. 中国计量科学研究院化学计量与分析科学研究所,北京,100013 |
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| 基金项目： | 国家质检总局食品安全专项资助项目，科技部基础性工作专项 |
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| 摘 要： | 为了满足食品及医药等领域的检测需求,研制了黄芩素纯度标准物质。采用液相色谱–质谱法和红外光谱法对黄芩素纯度标准物质原料定性后,利用高效液相色谱法(HPLC)和定量核磁技术(Quantitative Nuclear Magnetic Resonance Spectroscopy,QNMR)对黄芩素的纯度进行了定值,并用HPLC法对黄芩素纯度标准物质进行了均匀性检验和稳定性考察。对定值结果的不确定度进行了评价,研制的黄芩素纯度标准物质的定值结果和扩展不确定度分别为98.8%,0.8%(k=2)。
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| 关 键 词： | 黄芩素 标准物质 高效液相色谱法 定量核磁技术 不确定度评定 |
Certification and Uncertainty Evaluation of Baicalein Purity Certified Reference Material |
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| Yao Huanfang
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Quan Can
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Liu Jun
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Hou Caixia.Certification and Uncertainty Evaluation of Baicalein Purity Certified Reference Material[J].Chemical Analysis And Meterage,2012,21(6):4-8. |
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| Authors: | Yao Huanfang Quan Can Liu Jun Hou Caixia |
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| Institution: | 1. School of Chemical Engineering, Hebei United University, Tangshan 063009, China; 2. Division of Chemical Metrology and Analytical Science National Institute ofMetrology, Beijing 100013, China) |
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| Abstract: | To meet the requirement of detection field of medicine and food, the baicalein purity certified reference material （CRM） was developed. The qualitative analysis of baicalein purity CRM material was performed by liquid chromatography-mass spectrometry and infrared spectroscopy. The purity of baicalein was determined by high performance liquid chromatography （HPLC） and quantitative nuclear magnetic resonance spectroscopy （ QNMR ）. Homogeneity and stability monitoring of baicalein purity CRM were performed by HPLC. The uncertainty evaluation was studied extensively. The certified purity value of baicalein purity CRM was 98.8% with expanded uncertainty of 0.8 %（k=2）. |
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| Keywords: | baicalein certified reference material high performance liquid chromatography quantitative nuclear magnetic resonance spectroscopy uncertainty evaluation |
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