| 疣荔枝螺微粒体细胞色素P450酶系的光谱分析 |
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| 引用本文： | 韩雅莉,邓瑞鹏,周小朋.疣荔枝螺微粒体细胞色素P450酶系的光谱分析[J].光谱学与光谱分析,2006,26(1):130-133. |
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| 作者姓名： | 韩雅莉 邓瑞鹏 周小朋 |
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| 作者单位： | 1. 广东工业大学轻工化工学院,广东,广州,510090
2. 汕头大学生物系,广东,汕头,515063 |
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| 摘 要： | 从疣荔枝螺内脏团制备得到微粒体,用UV mini-1240分光光度计对细胞色素P450酶系的三种主要成分:细胞色素P450、细胞色素b5,NADPH-细胞色素P450还原酶进行了测定,并对微粒体酶液进行SDS-PAGE电泳测试,证明了疣荔枝螺体内含有细胞色素P450酶系的三种酶.
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| 关 键 词： | 疣荔枝螺 微粒体 细胞色素P450酶系 |
| 文章编号： | 1000-0593（2006）01-0130-04 |
| 收稿时间： | 2004-06-16 |
| 修稿时间： | 2004-09-28 |
Spectral Analysis and Study of the Microsome Cytochrome P450 Aromatase of Thais clavigera |
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| HAN Ya-li,DENG Rui-peng,ZHOU Xiao-peng.Spectral Analysis and Study of the Microsome Cytochrome P450 Aromatase of Thais clavigera[J].Spectroscopy and Spectral Analysis,2006,26(1):130-133. |
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| Authors: | HAN Ya-li DENG Rui-peng ZHOU Xiao-peng |
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| Institution: | 1. Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510090, China; 2. Department of Biology, Shantou University, Shantou 515063, China |
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| Abstract: | The microsomes were prepared from the viscera masses of Thais clavigeras. It is well known that there are mine main components in microsome cytochrome P450 aromatase, including cytochrome P450, cytochrome b5 and NADPH-cytochrome P450 reductase. So the contents of cytochrome P450 and cytochrome b5, and the activity of NADPH-cytochrome P450 reductase were determined by Shimadzu UV Visible spectrophotometer. And the mierasomal proteins were analysed by SDS-PAGE.All the results indicated that there exists P450 arom in the T. clavigeras. |
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| Keywords: | Thais clavigera Microsome Cytochrome P450 aromatase |
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