| 示差脉冲伏安法测定乳酸脱氢酶活性及纳米微粒生物毒性检测新方法研究 |
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| 引用本文： | 姚开安,王娜,赵斌,程炯佳,倪海燕,杨正标,孙成,毕树平.示差脉冲伏安法测定乳酸脱氢酶活性及纳米微粒生物毒性检测新方法研究[J].无机化学学报,2007,23(6):981-986. |
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| 作者姓名： | 姚开安 王娜 赵斌 程炯佳 倪海燕 杨正标 孙成 毕树平 |
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| 作者单位： | 南京大学污染控制与资源化国家重点实验室,生命分析化学教育部重点实验室,化学化工学院,南京,210093 |
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| 基金项目： | 国家自然科学基金;江苏省自然科学基金;高等学校博士学科点专项科研项目 |
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| 摘 要： | 采用示差脉冲伏安法，在乳酸脱氢酶(LDH)酶促体系“丙酮酸盐 + NADH +H+ (?) 乳酸盐 + NAD+”中，通过检测NAD+还原峰电流的变化，测定了不同条件下(不同酶用量、缓冲液pH值以及温度)LDH的活性、酶促体系的米氏常数KmNADH以及最大反应速率vmax。并且在最佳实验条件下，通过检测LDH活性的改变，实验考察了3种纳米物质(ZnS，TiO2(R)和TiO2(A))对乳酸脱氢酶酶促体系的影响。
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| 关 键 词： | 示差脉冲伏安法 乳酸脱氢酶 酶活性 纳米微粒 |
| 文章编号： | 1001-4861（2007）06-0981-06 |
| 修稿时间： | 2006-12-302007-04-30 |
Differential Pulse Voltammetric Studies on Lacate Dehydrogenase Activity and Method for Detection of Toxicity of Nanoparticles |
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| YAO Kai-An,WANG N,ZHAO Bin,CHENG Jiong-Ji,NI Hai-Yan,YANG Zheng-Biao,SUN Cheng and BI Shu-Ping.Differential Pulse Voltammetric Studies on Lacate Dehydrogenase Activity and Method for Detection of Toxicity of Nanoparticles[J].Chinese Journal of Inorganic Chemistry,2007,23(6):981-986. |
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| Authors: | YAO Kai-An WANG N ZHAO Bin CHENG Jiong-Ji NI Hai-Yan YANG Zheng-Biao SUN Cheng and BI Shu-Ping |
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| Institution: | State Key Laboratory of Pollution Control and Resource Reuse, MOE Key Laboratory for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093,State Key Laboratory of Pollution Control and Resource Reuse, MOE Key Laboratory for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093,State Key Laboratory of Pollution Control and Resource Reuse, MOE Key Laboratory for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093,State Key Laboratory of Pollution Control and Resource Reuse, MOE Key Laboratory for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093,State Key Laboratory of Pollution Control and Resource Reuse, MOE Key Laboratory for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093,State Key Laboratory of Pollution Control and Resource Reuse, MOE Key Laboratory for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093,State Key Laboratory of Pollution Control and Resource Reuse, MOE Key Laboratory for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 and State Key Laboratory of Pollution Control and Resource Reuse, MOE Key Laboratory for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093 |
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| Abstract: | The differential-pulse voltammetry (DPV) was used to study the lactate dehydrogenase (LDH) activity, KmNADH and vmax in the enzyme promoted catalytic reaction of "pyruvate NADH H lactate NAD " obtained by monitoring DPV reduction current of NAD . The effects of concentration of LDH, pH values and temperature on the LDH reaction system were investigated. Under the optimum experimental conditions, the influences of nanoparticles (ZnS, TiO2(R) and TiO2(A)) on the LDH activity as well as the whole enzyme promoted reaction system were studied. |
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| Keywords: | differential-pulse voltammetry lactate dehydrogenase activity of enzyme nanoparticles |
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