| 硝酸还原酶在巯基自组装膜上的电化学性质 |
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| 引用本文： | 宋雅茹,吴红,邵会波. 硝酸还原酶在巯基自组装膜上的电化学性质[J]. 电化学, 2002, 8(3): 333-336 |
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| 作者姓名： | 宋雅茹 吴红 邵会波 |
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| 作者单位： | 北京师范大学化学系,北京师范大学化学系,首都师范大学化学系 北京100875,北京100875,北京100037 |
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| 基金项目： | 国家自然科学基金 (2 99730 2 6 )，北京市自然科学基金 (2 992 0 0 7)，教育部高等学校骨干教师资助计划资助 |
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| 摘 要： | 应用自组装技术将硝酸还原酶 (NR)构筑在硫醇自组装单分子膜上 ,研究了Au/半胱胺 /NR和Au/半胱胺 /NR/卵磷脂两种酶电极在磷酸缓冲液中的直接电化学行为
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| 关 键 词： | 硝酸还原酶 卵磷脂 半脘胺自组装膜 |
| 文章编号： | 1006-3471(2002)03-0333-04 |
| 收稿时间： | 2002-08-28 |
| 修稿时间： | 2001-12-17 |
Electrochemical Properties of Nitrate Immobilized on Gold Electrodes with 2-aminoethanethiol SAMs |
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| SONG Ya_ru ,WU Hong ,SHAO Hui_bo. Electrochemical Properties of Nitrate Immobilized on Gold Electrodes with 2-aminoethanethiol SAMs[J]. Electrochemistry, 2002, 8(3): 333-336 |
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| Authors: | SONG Ya_ru WU Hong SHAO Hui_bo |
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| Affiliation: | SONG Ya_ru 1,WU Hong 1,SHAO Hui_bo 2* |
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| Abstract: | Nitrate reductase (NR) and phospholipids were immobilized on gold electrodes with 2_aminoethanethio(AET) SAMs. After Au/AET/NR and Au/AET/NR/lipid were soaked in pH 7 phosphate buffers for days,a pair of pre_reversible peak appeared at around 0.202 V and 0.149 V,respsctively, irregularly grew with soaking time in blank buffer.The cathodic peak current(i pcl )of Au/AET/NR system had linear relationship with scan rate between 0.02 and 0.5 V.s -1 ,while i pcl was proportional to V 1/2 in Au/AET/NR/lipid system.The direct electron transfer of enzyme electrodes was strongly affected by pH.Compared with Au/AET,NR exhibited a higher electrochemical activity in Au/AET/lipid system. |
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| Keywords: | Nitrate reductase Phospholipid 2_aminoethanethiol SAMs |
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