Detecting DNA methylation through changes in transverse proton relaxation |
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| Authors: | Jaganathan Hamsa Wang Pengfei Klein Jonathon Lossie Amy C Ivanisevic Albena |
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| Institution: | Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, USA. |
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| Abstract: | We present a facile, simple method to detect DNA methylation by measuring the transverse proton relaxation behaviour. Positively charged nanoparticles are arranged along the negatively charged backbone of DNA strands through electrostatic interactions. The arrangement of NPs along DNA strands aids to amplify and compare the transverse proton relaxation signal for un-cut versus cut DNA strands cleaved by sequence specific restriction enzymes. Results from this study suggest that the presence of methylation on DNA can be detected using superparamagnetic NPs using NMR. |
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