Aptamer-based competitive electrochemical assay of small biomolecules |
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| Authors: | ZHANG YuYong XIANG Yun CHAI YaQin & YUAN Ruo Key Laboratory on Luminescence Real-Time Analysis Ministry of Education School of Chemistry Chemical Engineering Southwest University Chongqing China |
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| Institution: | ZHANG YuYong,XIANG Yun,CHAI YaQin & YUAN Ruo Key Laboratory on Luminescence and Real-Time Analysis,Ministry of Education,School of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,China |
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| Abstract: | A convenient aptamer-based competitive electrochemical biosensor for a small biomolecule,adenosine,was described. The sensing surface was fabricated by self-assembly of an aptamer/mercaptohexanol monolayer on a gold disk electrode. The principle of this aptasensor is based on the competition between an adenosine target molecule and a ferrocene-conjugated signaling DNA strand for the aptamer binding site on the sensing surface. Due to the competitive nature of this assay,the electrochemical responses of the ... |
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| Keywords: | aptamer adenosine biosensor competitive assay differential pulse voltammetry |
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