A nano-functionalized real-time electrochemiluminescent biosensor for alanine transaminase assay |
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| Authors: | CHU HaiHong WU MeiSheng CAI Xia & TU YiFeng Institute of Analytical Chemistry |
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| Affiliation: | CHU HaiHong,WU MeiSheng,CAI Xia & TU YiFeng Institute of Analytical Chemistry,Department of Chemistry,Soochow University,Suzhou 215123,China |
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| Abstract: | An electrochemiluminescent (ECL) biosensor was constructed for selective assay of alanine aminotransferase (ALT) based on the enzymatically catalyzed oxidation of pyruvate by pyruvate oxidase (PYOD). The composite of potassium ferricyanide and carbon nanotube was adopted to pre-functionalize the basal platinum electrode while the potassium ferricyanide acted as the activator of PYOD. The ALT catalyzed the reaction of L-alanine and-ketoglutarate to produce pyruvate which could be further enzymatically oxidiz... |
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| Keywords: | alanine aminotransferase electrochemiluminescent biosensor luminol pyruvate oxidase carbon nanotubes |
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