Efficient electroformation of supergiant unilamellar vesicles containing cationic lipids on ITO-coated electrodes |
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Authors: | Herold Christoph Chwastek Grzegorz Schwille Petra Petrov Eugene P |
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Institution: | Biophysics, BIOTEC, Technische Universit?t Dresden, Dresden, Germany. |
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Abstract: | Giant unilamellar vesicles (GUVs) represent a versatile in vitro system widely used to study properties of lipid membranes and their interaction with biomacromolecules and colloids. Electroformation with indium tin oxide (ITO) coated coverslips as electrodes is a standard approach to GUV production. In the case of cationic GUVs, however, application of this approach leads to notorious difficulties. We discover that this is related to aging of ITO-coated coverslips during their repeated use, which is reflected in their surface topography on the nanoscale. We find that mild annealing of the ITO-coated surface in air reverts the effects of aging and ensures efficient reproducible electroformation of supergiant (diameter > 100 μm) unilamellar vesicles containing cationic lipids. |
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